The secondary structure of compounds 1 to 5 (0.1 mg/ml in PBS) was analyzed by CD. Spectra were tested with the following parameters: 5-nm bandwidth; scan rate, 50 nm/min; response time, 2 s; and 1-nm intervals over the wavelength range of 195 to 260 nm in a nitrogen atmosphere. Reported data are the mean of six accumulations. Mean residue molar ellipticity (deg∙cm2∙dmol−1) was calculated using the formula [θ] = mdeg/(l × c × n) * 1000, where l is the path length (0.1 cm), c is the peptide concentration (millimolar), and n is the number of residues in the peptide.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.