The enzyme assays for 20 cell cycle–related kinases were conducted by the ChemPartner company (Shanghai, China) according to standard mobility shift assay protocols. Briefly, the diluted compounds were performed in prepared kinase buffer with ATP solution. Then, all samples were transferred to each well of a 384-well plate for incubation at room temperature for 10 min. The data were collected after adding stop buffer to stop reaction. The IC50 values were calculated by fitting the data points with the dose-response function in GraphPad Prism 6.0 (GraphPad Software) from three independent experiments.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.