The enzyme assays for 20 cell cycle–related kinases were conducted by the ChemPartner company (Shanghai, China) according to standard mobility shift assay protocols. Briefly, the diluted compounds were performed in prepared kinase buffer with ATP solution. Then, all samples were transferred to each well of a 384-well plate for incubation at room temperature for 10 min. The data were collected after adding stop buffer to stop reaction. The IC50 values were calculated by fitting the data points with the dose-response function in GraphPad Prism 6.0 (GraphPad Software) from three independent experiments.

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