In general, the ATP binding site competitive FP assay was performed as previously reported (22). FP values were determined on a SpectraMax multimode microplate reader (Molecular Devices SpectraMax Paradigm; excitation, 485 nm; emission, 535 nm). The final inhibition ratio was calculated by GraphPad Prism 6.0, and the values of the inhibition rate were determined as previously reported (37).

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