Tissues were fixed, embedded in paraffin, and mounted on glass slides. After deparaffinization and rehydration, slides were incubated with 0.2% glycine for 5 min to quench autofluorescence, followed by incubation with various antibodies (10 to 20 μg/ml) at 4°C for 18 hours. PLA was performed using the Duolink in situ hybridization kit as described by the manufacturer (Sigma-Aldrich). Images were quantified by Duolink Image Tool Software (Olink Bioscience).

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