Differential gene expression experiments were performed on a carbapenem- and tigecycline-resistant clinical A. baumannii isolate (P1270). This isolate was exposed to 0.1×, 0.25×, and 0.5× MIC concentrations of Ω76. A control cohort containing untreated A. baumannii (P1270) was also used. All samples were incubated at 37°C for 12 hours in 10 ml of Mueller Hinton broth supplemented with meropenem (8 mg/liter) to maintain the resistant phenotype. After incubation, these four samples were pelleted down at 6000 rpm for 10 min and flash-frozen with liquid N2. Genotypic (India) performed quality control, RNA extraction, and A. baumannii microarray mRNA hybridization experiments using an Agilent CGH platform (Agilent A. baumannii_8X15k_GXP AMADID: 7936). Two technical replicates were performed for every sample, resulting in eight total samples.

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