Total RNA isolation was performed using RNeasy (Qiagen), and 1 μg of total RNA was used for complementary DNA (cDNA) synthesis using the iScript cDNA Synthesis Kit (Bio-Rad). TaqMan probes (CerS1, Hs00162669; CerS2, Hs00184211; CerS4, Hs00219999; CerS5, Hs00908758_g1; and CerS6, Hs00831972) were used for quantitative PCR (qPCR) (Thermo Life Technologies).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.