Total RNA isolation was performed using RNeasy (Qiagen), and 1 μg of total RNA was used for complementary DNA (cDNA) synthesis using the iScript cDNA Synthesis Kit (Bio-Rad). TaqMan probes (CerS1, Hs00162669; CerS2, Hs00184211; CerS4, Hs00219999; CerS5, Hs00908758_g1; and CerS6, Hs00831972) were used for quantitative PCR (qPCR) (Thermo Life Technologies).

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