The H-I injury was performed as previously described (6). Briefly, P8 mice were anesthetized with isoflurane, and the left common carotid artery was permanently ligated with 6-0 silk sutures and then transected. Mice were returned to their home cage to recover for 1.5 hours. Subsequently, mice were placed in a hypoxic chamber (8% oxygen) for 1 hour while the body temperature was maintained at 37°C using a heating pad. Castration and ovariectomy surgeries were performed by the Jackson laboratory at P28 before arrival.

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