All experiments were performed in accordance with institutional guidelines approved by the Animal Care Committee at the University of Toronto. C57Bl/6 mice were purchased from Charles River Laboratories (Montreal, QC) or bred in-house. Castrated and ovariectomized mice were purchased from the Jackson laboratory (Maine, USA). Rosa-YFP mice used for the coculture experiments were bred in-house. Following weaning, mice were group housed in a 12-hour light/dark cycle, and food and water were provided ad libitum. Throughout these experiments, neonatal mice were defined as P8 to P12, juvenile mice were P17 to P28, and adults were over 6 weeks of age.

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