The Transport of Diluted Species module of COMSOL Multiphysics was used to numerically solve the coupled solute and particle conservation equations (details in the Supplementary Materials). The microfluidic device and beacon dimensions used for the computations were directly obtained from experimental measurements, and other physical parameters such as the diffusion coefficient of SDS were obtained from the literature. Space was meshed with a triangular mesh, and solute and particle concentration profiles were calculated. Results were then exported in matrix form and postprocessed in R to obtain radial (Vr) and azimuthal (Vφ) particle velocities.

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