Lactate concentration was measured to evaluate the anaerobic metabolism on which animals rely during hypoxia exposure (34), using a Lactate Pro 2 Analyser (www.lactatepro.com.au/) on the hemolymph of the venous blood withdrawn from the sinus below the arthrodial membrane, at the base of the fourth or fifth pereiopod of the crab [see methods in (35)]. Lactate was tested in 38 animals exposed to 10% of water oxygen saturation for 15 hours, obtained by flushing nitrogen into the seawater. We then placed 19 animals under normoxia and 19 at hyperoxia, and after 1 hour and 45 min, we remeasured lactate to assess the efficiency of the recovery from lactate accumulation during hypoxia exposure. This measure was made at a constant temperature of 25°C. Water oxygen saturation was monitored using a MiniDOT logger (www.pme.com/products/minidot).

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