Total RNA from three embryos was extracted using TRIzol (Invitrogen), and cDNA synthesis was carried out using random hexamer priming and the StrataScript Reverse Transcriptase. Quantitative reverse transcription PCR was performed on the 7900HT Fast Real-Time PCR TaqMan System (Applied Biosystems) using the Brilliant SYBR Green qPCR Master Mix (Stratagene). Measurements were performed in triplicates and normalized to the expression levels of eukaryotic translation elongation factor 1 α (ef1a). Bars indicate SE. The primer sequences are described in data file S1.

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