Total RNA from three embryos was extracted using TRIzol (Invitrogen), and cDNA synthesis was carried out using random hexamer priming and the StrataScript Reverse Transcriptase. Quantitative reverse transcription PCR was performed on the 7900HT Fast Real-Time PCR TaqMan System (Applied Biosystems) using the Brilliant SYBR Green qPCR Master Mix (Stratagene). Measurements were performed in triplicates and normalized to the expression levels of eukaryotic translation elongation factor 1 α (ef1a). Bars indicate SE. The primer sequences are described in data file S1.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.