Before enzyme activity measurements, samples were subjected to inductively coupled plasma optical emission spectrometry (ICP-OES) analysis with an ICP-OES 5110 instrument (Agilent) to ascertain metal content. qNOR activity was measured using a Clark-type electrode fitted with an ISO-NO Mark II system (WPI). All reaction components (aside from glucose oxidase and catalase) were made anaerobic by replacing the atmosphere of the vials with N2. The assay buffer contained 50 mM Na-citrate (pH 6.0), 0.05% DDM or DTM, 100 mM d-glucose, 10 μg ml−1 glucose oxidase, and 10 μg ml−1 catalase. Glucose, glucose oxidase, and catalase were added to scavenge any oxygen left in the reaction vessel. Sodium ascorbate (1 mM) and phenazine methosulfate (10 μM) acted as an electron donation system. A 2 mM NO saturated solution [50 mM Na-citrate (pH 6.0)] was made and added at a final concentration of 20 μM. NO consumption was started by addition of the protein at a final concentration of 0.2 μM.

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