Two-cell stage embryos were injected into one blastomere with 10 ng of Bap1MO and maintained in 4% Ficoll/1× MMR solution at 22°C until maturing to stage 6. SAHA was dissolved in 100% dimethyl sulfoxide (DMSO) to make a 1 mM stock solution and kept at −20°C. SAHA was added to medium containing the embryos to a final concentration of 20 μM. Control sibling embryos were treated with an equal volume of DMSO. Upon reaching stage 10, embryos were transferred to 0.1 MMR solution supplemented with fresh SAHA every 4 hours. Embryos were collected and fixed at different stages for morphological and molecular analysis.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.