Two-cell stage embryos were injected into one blastomere with 10 ng of Bap1MO and maintained in 4% Ficoll/1× MMR solution at 22°C until maturing to stage 6. SAHA was dissolved in 100% dimethyl sulfoxide (DMSO) to make a 1 mM stock solution and kept at −20°C. SAHA was added to medium containing the embryos to a final concentration of 20 μM. Control sibling embryos were treated with an equal volume of DMSO. Upon reaching stage 10, embryos were transferred to 0.1 MMR solution supplemented with fresh SAHA every 4 hours. Embryos were collected and fixed at different stages for morphological and molecular analysis.

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