Morpholino antisense oligonucleotides were designed and synthesized against X. laevis bap1, asxl, and hdac4, targeting both homologs, from the long and short chromosomes (Gene Tools LLC). Morpholino against hdac3 was designed as previously described (data file S1). Doses of 5 to 15 ng per embryo were microinjected as mentioned above. Plasmids containing cDNA clones that were used to generate WISH RNA probes were obtained from previously described sources (data file S1). The following constructs were purchased from GE Dharmacon (Lafayette, CO): asxl1 (ID446414), bap1 (ID493568), and fzd7 (ID378787). Mutations were introduced to Xenopus bap1 and asxl1 cDNA using a Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA).

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