Rats were slightly anesthetized with isoflurane. Blood samples (0.5 ml) were taken via jugular vein before (baseline) and 40 min and via abdominal vein 70 min after administration of yohimbine (5 mg/kg, intraperitoneally). Acupuncture was given 30 min after an intraperitoneal administration of yohimbine. Blood samples were collected in EDTA coating tube and centrifuged at 3500g for 10 min, and plasma was stored in refrigerator until analyzed. Plasma corticosterone levels were quantified using an ELISA kit (ADI-900-097, Enzo Life Sciences Inc., USA).

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