The 4T1 or A375 cells were seeded into confocal dishes and treated with various samples when the cell confluence reached 70%, which include TCPS, ACC-CaSi-PAMAM-FA/mPEG, DOX, ACC@DOX-CaSi-PAMAM-FA/mPEG, ACC@DOX.Fe2+-CaSi-PAMAM-FA/mPEG, and MMP-2–pretreated ACC@DOX.Fe2+-CaSi-PAMAM-FA/mPEG. After incubation for 12 hours, the tumor mitochondria were stained with JC-1 dye following the procedures provided in the user manual and then observed by CLSM.

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