The following drugs were used: β-endorphin [a μ-opioid receptor agonist; 0.25 and 0.5 μg per side, dissolved in sterile saline (site), Bachem, Switzerland]; yohimbine (an anxiogenic compound; 5 mg/kg, dissolved in distilled water, intraperitoneal injection; Sigma-Aldrich, USA); naloxone (a nonselective opioid receptor antagonist; 1.0 mg/kg, dissolved in sterile saline, intraperitoneal injection; Sigma-Aldrich, USA); harmaline (a tremogenic compound; 10 mg/kg, dissolved in sterile saline, subcutaneous injection; Sigma-Aldrich, USA); and a retrograde tracer, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine (DiI) (2%, dissolved in methanol, Life Technologies, USA).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.