Adenosine levels were measured from plasma of BM flush or cultured media using adenosine assay kit (Abcam), according to the manufacturer’s instructions. For BM measurements, femur and tibia containing marrow were centrifuged at 200g for 1 min at 4°C to collect whole-marrow flush. The flush was then centrifuged at 2000g for 5 min at 4°C to separate the cell and plasma fractions. For cell media measurements, media cultured with cells for 3 days were collected. The BM plasma or cell media samples were then diluted and mixed with reaction mix containing adenosine assay buffer, adenosine detector, adenosine converter, adenosine developer, and adenosine probe at 1:1 ratio in a well of 96-well white plate. Fluorescence intensity was detected with a plate reader (Tecan Infinite 200 PRO) using Ex535 and Em590 nm filters. Fluorescence was subtracted from background and quantified using a standard.

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