To coat the thin silica shell onto the ACC@DOX.Fe2+, 2 mg of ACC-DOX.Fe2+ was first dispersed into 20 ml of ethanol and then added with 25 μl of EDTA (12 mg/ml) and 400 μl of ammonia (25%, v/v). After stirring the mixture at 400 rpm for 15 min, 30 μl of TEOS and CaCl2 was added, followed by 400 μl of deionized water. The reaction would continue for another 24 hours, and the product was denoted as ACC@DOX.Fe2+-CaSi.

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