Synthesis of PAMAM-FA: To start with, FA (79.4 mg, 0.18 mmol) was dissolved in 10 ml of N,N′-dimethylformamide (DMF). Subsequently, EDC (0.4 mmol, 77.8 mg) and NHS (0.4 mmol, 46 mg) were added into the solution and stirred for 30 min to activate the terminal carboxyl groups. After the activation was complete, PAMAM (100 mg, 0.14 mmol) was added and the reaction lasted for 24 hours under stirring. The intermediate product was extracted via dialysis (molecular weight cut-off (MWCO): 1000 Da Millipore) and lyophilization and denoted as PAMAM-FA.

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