Synthesis of PAMAM-FA: To start with, FA (79.4 mg, 0.18 mmol) was dissolved in 10 ml of N,N′-dimethylformamide (DMF). Subsequently, EDC (0.4 mmol, 77.8 mg) and NHS (0.4 mmol, 46 mg) were added into the solution and stirred for 30 min to activate the terminal carboxyl groups. After the activation was complete, PAMAM (100 mg, 0.14 mmol) was added and the reaction lasted for 24 hours under stirring. The intermediate product was extracted via dialysis (molecular weight cut-off (MWCO): 1000 Da Millipore) and lyophilization and denoted as PAMAM-FA.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.