Shuar circulating concentration of IgG was measured using a validated enzyme-linked immunosorbent assay protocol in finger-prick dried blood spot (DBS) samples (7). Participant DBS samples were collected following REE measurement on days 0, 7, and 14 using standard procedures (38). All DBS samples were stored at −20°C via a solar-powered freezer until shipped to the United States for storage at −30°C. Samples were measured for IgG in duplicate with two-level controls. Intra- and interassay measurement CVs for the assay are 2.0 to 2.7% and 8.0 to 10.8%, respectively (7). Final IgG concentration was obtained by averaging participant repeated weekly measures.

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