The DLW method is the gold standard for the measurement of free-living TEE in humans (37). DLW measures of TEE and FFM are directly comparable across laboratories following standardized data collection and analytical protocols (37). To provide additional assurance that our CRDS DLW measures were reliable and directly comparable to U.S./U.K. values, duplicate measures were obtained for six Shuar participants using isotope-ratio mass spectrometry at an external laboratory (between assay CVTEE = 2.0%, CVFFM = 0.3%; table S6).

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