All procedures were performed as described in the manual supplied with the luciferase assay system from Promega. HEK293T cells were seeded in a 12-well plate the day before transfection. The cells were subsequently cotransfected with the luciferase reporter pGL3E-pViperin, pRL-TK, and the FLAG-tagged RTA plasmid or the FLAG-tagged empty vector plasmid. The pRL-TK plasmid was used to normalize firefly luciferase activity based on the expression of Renilla luciferase activity. The cells were lysed in 200 μl of passive cell lysis buffer at 48 hours after transfection, and luciferase activity was detected. The results were expressed as the fold change relative to cells transfected with the FLAG-tagged empty vector plasmid.

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