Mass spectrometry was used to identify KSHV helicase and MCM7 protein complexes. 293T cells transfected with the indicated plasmids using Lipofectamine 2000, and KSHV helicase-FLAG recombinant virus–infected cells were induced with doxycycline for 72 hours. M2-FLAG beads and 3× FLAG peptides were used to purify native protein complexes and elute beads, respectively, from 293T cells. Protein A/G beads and an anti-FLAG antibody were applied to related KSHV helicase–FLAG recombinant virus–infected cells. The eluate was monitored via SDS–polyacrylamide gel electrophoresis (PAGE) as well as silver staining kit and lastly subjected to mass spectrometry. Besides, mass spectrometry was also used to analyze methionine oxidation of KSHV helicase, MCM7, and RIG-I.

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