Human embryonic kidney (HEK) 293T, MEF, iSLK-puro, iSLK-RGB, and related KSHV helicase mutation recombinant virus–infected cell lines were maintained in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), antibiotics (penicillin and streptomycin), and proper selective agents [hygromycin (0.5 mg/ml), puromycin (1.5 μg/ml), and G418 (0.5 mg/ml)]. All cells were cultured at 37°C in the presence of 5% CO2.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.