Human embryonic kidney (HEK) 293T, MEF, iSLK-puro, iSLK-RGB, and related KSHV helicase mutation recombinant virus–infected cell lines were maintained in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), antibiotics (penicillin and streptomycin), and proper selective agents [hygromycin (0.5 mg/ml), puromycin (1.5 μg/ml), and G418 (0.5 mg/ml)]. All cells were cultured at 37°C in the presence of 5% CO2.

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