Animal model was built up and analyzed depending on Institutional Animal Use and Care Committee at Chinese Academy of Sciences. About 4- to 6-week-old female nude mouse was injected with cancer cells subcutaneously. After the tumors were detectable, the mice were divided into some groups randomly, which was depending on the tumor volume (length × width2/2) of each mouse. Then, the xenografts were treated by different chemicals, PBS or Dox. After being treated for several days, the mice were euthanized, while each organ or tumor was collected for further analysis.

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