About 3 × 105 cells were grown on the glasses of each well at 37°C overnight and then precultured in a hypoxic incubator for about 12 hours at 37°C. After being treated with drug materials for the indicated time under hypoxia condition, cells were washed orderly with ice-cold PBS and distilled H2O quickly, immersed into liquid nitrogen, and transferred into a freeze dryer immediately. After being dried overnight, each glass was coated with gold at 2 nm thick and then visualized.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.