Cells were grown in six-well plates for about 12 hours before transfection experiments were carried out. After small interfering RNA (siRNA) and transfection reagent were mixed well in Opti-MEM, cells were transfected with this mixture for about 6 hours. Then, Opti-MEM was replaced with complete medium. Being cultured overnight, cells were exposed to different treatments. Lipofectamine 2000 transfection reagent was purchased from Thermo Fisher Scientific, while siRNA sequence (5′-GGAAGAAAUCGCUGAGGAA-3′) and its negative control were from GenePharma.

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