Cells were grown on the surface of glasses and treated with drug materials for the indicated time. Before incubation with primary antibodies at 4°C overnight, cells were fixed with 4% paraformaldehyde, treated with 0.1% Triton X-100, and blocked with 2% bovine serum albumin (BSA). After being washed with phosphate-buffered saline (PBS), each sample was incubated with secondary antibodies at room temperature for about 2 hours. After that, each sample underwent a second round of washes and was treated with 4′,6-diamidino-2-phenylindole staining buffer for about 5 min. Last, slides were washed and mounted with the antifade medium immediately.

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