Four different samples were prepared for CD analysis, including 0.75 wt % of N-pepABS hydrogels at pH 5.0 or pH 7.0, and diluted in N-pepABS solutions (0.01 wt %) and in methanol (0.005 wt %) at pH 9.0. Then, they were analyzed using JASCO J-1500 in 1-mm quartz cuvette. With a bandwidth set to 2 nm and a scanning speed set to 200 nm/min, all spectra have been collected in an average of three scans. The parameter of high tension value was kept between 200 and 650 during all measurements.

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