N-pep (0.3 mmol) was added to a mixture of methanol (6 ml) and ddH2O (1 ml), followed by adding 1 M NaOH to adjust the pH value to 8, and iABS (0.33 mmol) was dissolved in methanol (1 ml) and added to the solution. The mixture was stirred overnight at room temperature. The resulted crude product was purified by reverse-phase HPLC. 1H NMR (400 MHz, DMSO-d6): δ 8.25 (d, J = 8.1 Hz, 1H), 8.13 (d, J = 8.3 Hz, 1H), 7.85 (d, J = 7.4 Hz, 1H), 7.80 to 7.76 (m, 1H), 7.75 (d, J = 2.9 Hz, 2H), 7.73 (d, J = 3.5 Hz, 2H), 7.58 (s, 1H), 7.50 to 7.44 (m, 2H), 7.43 (d, 2H), 7.30 to 7.12 (m, 10H), 4.61 to 4.55 (m, 2H), 4.54 to 4.46 (m, 1H), 4.20 (dd, 1H), 3.59 (s, 2H), 3.53 (dd, J = 35.9, 14.0 Hz, 2H), 3.08 to 3.03 (m, 2H), 2.95 to 2.79 (m, 2H), 2.69 (dd, J = 13.4, 10.3 Hz, 1H), and 1.33 to 1.64 (m, 6H). Mass spectometry (electrospray ionization) (mass/charge ratio): C45H51N6O7S2 calculated 850.32; found 851.33 [M + 1]+.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.