N-pep (0.3 mmol) was added to a mixture of methanol (6 ml) and ddH2O (1 ml), followed by adding 1 M NaOH to adjust the pH value to 8, and iABS (0.33 mmol) was dissolved in methanol (1 ml) and added to the solution. The mixture was stirred overnight at room temperature. The resulted crude product was purified by reverse-phase HPLC. 1H NMR (400 MHz, DMSO-d6): δ 8.25 (d, J = 8.1 Hz, 1H), 8.13 (d, J = 8.3 Hz, 1H), 7.85 (d, J = 7.4 Hz, 1H), 7.80 to 7.76 (m, 1H), 7.75 (d, J = 2.9 Hz, 2H), 7.73 (d, J = 3.5 Hz, 2H), 7.58 (s, 1H), 7.50 to 7.44 (m, 2H), 7.43 (d, 2H), 7.30 to 7.12 (m, 10H), 4.61 to 4.55 (m, 2H), 4.54 to 4.46 (m, 1H), 4.20 (dd, 1H), 3.59 (s, 2H), 3.53 (dd, J = 35.9, 14.0 Hz, 2H), 3.08 to 3.03 (m, 2H), 2.95 to 2.79 (m, 2H), 2.69 (dd, J = 13.4, 10.3 Hz, 1H), and 1.33 to 1.64 (m, 6H). Mass spectometry (electrospray ionization) (mass/charge ratio): C45H51N6O7S2 calculated 850.32; found 851.33 [M + 1]+.

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