To synthesize pepABS, Fmoc-(d)-Phe-(d)-Phe-(d)-Lys-OH was first gained by SPPS. Then, pure Fmoc-(d)-Phe-(d)-Phe-(d)-Lys-OH (0.2 mmol) was added to a mixture of methanol (4 ml) and ddH2O (1 ml), followed by adding 1 M NaOH to adjust the pH value to 8. Isothiocyanate-benzensulfonamide (iABS) (0.22 mmol) was dissolved in methanol (1 ml) and added to above solution. The mixture was stirred overnight at room temperature. After purification by HPLC, dry powder of Fmoc-(d)-Phe-(d)-Phe-(d)-Lys-(benzenesulfonamide)-OH (0.1 mmol) was collected and then dissolved in 20% (v/v) piperidine in DMF (2 ml) and reacted for 30 min. The resulting crude products were purified by reverse-phase HPLC to gain white powder. 1H NMR (400 MHz, DMSO-d6): δ 8.48 (d, J = 7.7 Hz, 1H), 8.01 (d, J = 5.3 Hz, 3H), 7.76 (d, J = 8.3 Hz, 2H), 7.50 (s, 2H), 7.42 (d, 2H), 7.39 to 7.17 (m, 10H), 4.67 (t, J = 8.8, 4.3 Hz, 1H), 4.22 (t, J = 8.3, 5.0 Hz, 1H), 4.01 (s, 1H), 3.20 to 3.03 (m, 2H), 2.98 to 2.78 (m, 4H), 2.6 (s,2H), and 1.80 to 1.32 (m, 6H).

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