Corneal sensitivity of mouse eyes was measured by a manual esthesiometer (12/100 mm; Luneau SAS, France). The esthesiometer consisting of a nylon filament 6 cm in length is applied to the center of the mouse cornea, and the pressure exerted was measured as blink response. At the highest length, if the mice blinked, it was considered most responsive, and the absence of a blink at the shortest length was considered least responsive. The length of the nylon filament was reduced 0.5 cm at a time to record the blink response. The measurement was taken in triplicate at 10-s intervals. The mice that did not respond were given an arbitrary score of 0. A high score indicates high/normal corneal sensitivity, while a low score corresponds to the absence or loss of corneal sensitivity.

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