C57BL/6 mice, bred and housed at the university biological resource laboratory, were used for the ocular model of murine HSV-1 infection. Standard feed and water were provided to the mice with a 12-hour light/12-hour dark cycle with no more than five mice per cage. On day 0, 6- to 8-week-old mice were anesthetized, as described previously, before the application of a topical anesthetic (proparacaine hydrochloride, 0.5%). Corneal epithelial debridement was performed using a 30-gauge needle followed by the application of 5 × 105 PFU HSV-1 (McKrae) to the eye. Topical treatments were performed on days 1, 3, 5, 7, and 9 after infection, while ocular washes and mice pictures (Carl Zeiss Stereoscope) were collected on days 2, 4, 7, and 10 after infection.

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