ACV (100 μg), dissolved in 50 μl of DMSO, was added to HPAC (1 mg/ml) and incubated at room temperature overnight on a 3D rocker (BioRocker 3D, Denville, USA). The mixture was centrifuged at 14,000g for 15 min to separate the drug-loaded HPAC particles from the free drug supernatant. Supernatant was analyzed for the amount of ACV using the UV spectrophotometer, as mentioned above. Three individual loading experiments were conducted to analyze the drug loading efficiency, and as-prepared DECON particles were stored at 4°C for further characterization.

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