Cells infected with GFP virus were collected 24 hpi using Hanks’-based, enzyme-free cell dissociation buffer (Gibco, 13150). Cells were washed once with PBS before they were fixed using 4% parafolmaldehyde (Electron Microscopy Sciences, USA) and suspended in fluorescence-activated cell sorting (FACS) buffer (PBS and 5% FBS). Cell suspensions were filtered through a 70-μm mesh, and cells were resuspended in FACS buffer before analyzing them using an in-house flow cytometer (BD Accuri C6 Plus). A total of 4 × 104 cells were collected for each sample, and the analysis was performed using FlowJo (version 10).

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