Mock, neutralization, and prophylactic treatments similar to those mentioned in earlier sections were conducted with an MOI of 50 using GFP fluorescent viruses. The infection was allowed to occur for a period of 90 min at 4°C followed by a brief incubation of 15 min at 37°C. This process allowed the virus to remain on the cell surface rather than enter the cell. After 15 min of incubation, the cells were fixed using 4% paraformaldehyde followed by nuclear (4′,6-diamidino-2-phenylindole) and actin (rhodamine-phalloidin) staining. The cells were visualized to understand the extent of infection reaching the cell surface. Five images per sample were procured and quantitatively analyzed for GFP fluorescence per image. The quantitative data would directly translate to the total amount of viral infection in a given area. The fluorescent intensity of all of the channels was kept constant, and the images were analyzed using MetaMorph Microscopy Automation and Image Analysis Software to quantitatively determine total intensity from the green channel.

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