The harvested spheroids were placed in a Tyrode buffer solution–filled recording chamber attached to the 3D-SR-BA chip. Each 3D-SR-BA was unrolled using a micromanipulator (SMX, Sensapex), as illustrated in movie S2, followed by the spheroid encapsulation inside the arrays. After encapsulation, the recording chamber was perfused continuously with fresh Tyrode’s solution at 37°C.

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