The radius of curvature was estimated using the 3D reconstruction of confocal images in Nikon NIS Elements software. Briefly, the y-z plane slices of the z-stack were obtained to visualize the circumference of each 3D-SR-BA. The radii were measured by using a three-point circular fit to the 3D-SR-BA circumference slice in NIS Elements software, as illustrated in fig. S2A.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.