Statistical analysis was performed with Excel (Microsoft) and Prism software (GraphPad Software). Error bars represent means ± SEM. All representative experimental findings were verified in at least three independent experiments. Data were verified to be in a normal distribution. Results are expressed as mean ± SEM. Two-tailed Student’s t test was used to compare differences between groups. Analysis of variance (ANOVA) was applied to perform multiple comparisons. Mantel-Cox test was applied to compare survival rates between groups. P < 0.05 was considered statistically significant.

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