After fixation with 4% paraformaldehyde, the cell was investigated with a confocal Raman microscope (alpha300R, WITec, Ulm, Germany) using a 532-nm Nd:YAG laser. Spectrum was acquired with a peltier-cooled charge-coupled device detector (DV401-BV, Andor, UK) with 300 gratings/mm (UHTS 600, WITec, Germany). WITec suite (version 5.0, WITec, Germany) was used for data acquisition.

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