The primary anti-mouse antibodies used were cleaved caspase 3 (Asp175, catalog no. 9661, Cell Signaling Technology, Danvers, MA), ADAM10 (catalog no. AB19026, Merck Millipore, Darmstadt, Germany), ADAM17 (catalog no. AB19027, Merck Millipore), and β-actin (clone 8H10D10, catalog no. 3700, Cell Signaling Technology). The secondary antibody was a conjugated immunoglobulin G (IgG) (Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA). Quantification of bands was achieved by densitometry using the ImageJ software.

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