A handmade Al holder with a Si frame was attached under a 35-mm culture dish square hole in the center by double-sided tape, as previously described (44). The SE-ADM culture dish holder was subsequently ultraviolet-sterilized for 17 to 18 hours, and osteoblasts were cultured in the dish holder. Next, the Al holder containing cells was separated from the plastic culture dish, attached upside down to another SiN film on an acrylic plate, and sealed (15).

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