Human plasma sFgl2 was determined with a human Fgl2 ELISA kit (catalog no. 436907, BioLegend) according to the manufacturer’s instructions. Mouse peritoneal exudates, plasma, and cell supernatants were used for detecting sFgl2 (catalog no. 437808, BioLegend) and other cytokines [IL-1β, IL-10, MCP-1, CXCL1, IL-6, IL-12p40, TNF, and interferon-γ (IFN-γ); ELISA kits from R&D Systems, Minneapolis, MN, USA]. In some indicated experiments, 10 μM GI254023X (ADAM10 inhibitor; catalog no. SM20789, Sigma-Aldrich), 10 μM TMI-1 (ADAM17 inhibitor; catalog no. 287403-39-8, TOCRIS), 10 μM GW280264X (dual ADAM10 and ADAM17 inhibitor; catalog no. AOB3632, Aobious, Gloucester, MA), 10 μM H89 (PKA inhibitor; catalog no. S1582, SelleckChem, Houston, TX), 10 nM LXA4 (5S,6R,15S-TriHETE; catalog no. 90410, Cayman Chemical), 10 nM RvD1 (7S,8R,17S-trihydroxy-4Z,9E,11E,13Z,15E,19Z-docosahexaenoic acid; catalog no. 10012554, Cayman Chemical), 10 nM RvD2 (7S,16R,17S-trihydroxy-4Z,8E,10Z,12E,14E,19Z-docosahexaenoic acid; catalog no. 10007279, Cayman Chemical), 1 μM baicalein (catalog no. 491-67-8, Adamas), and 1 μM WRW4 (catalog no.344220, Calbiochem) were administrated. For the experiments of mFgl2 determination, cell proteins were collected with a Membrane and Cytoplasmic Protein Extraction kit (catalog no. C510005, Sangon Biotech, Shanghai, China) and assessed with ELISA (catalog no. 436907 for human and catalog no. 437808 for mouse; BioLegend) and were normalized with β-actin (PathScan Total β-Actin Sandwich ELISA Kit, #7880, Cell Signaling Technology).

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