The electrochemical characterization of the flexible sensor was evaluated in a 0.1 M PBS (pH 7.0) with change concentration of analyst. The AA and UA sensors were evaluated by DPV with oxidation of −0.08 V (versus Ag/AgCl) for AA and 0.21 V (versus Ag/AgCl) for UA. Chronoamperometry was used for the determination of lactate and glucose with an initial potential of −0.2 V (versus Ag/AgCl) for lactate and 0.65 V (versus Ag/AgCl) for glucose, respectively. As for Na+ and K+ sensors, direct recording of OCP from the two-electrode system was used. The LOD was defined as the lower concentration in which the response was three times the SD of noise. To investigate the reproducibility of the sensors, the electrochemical responses of five samples fabricated in different batches were compared. To investigate the stability of the sensors, the performance of five samples were recorded each week over a period of 4 weeks, and other times, the samples were stored at 4°C to preserve the activity of the enzyme on the sensor.

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