Extraction of the height transition from HS-AFM data

We extracted height transitions using a self-written program based on Igor Pro (Wavemetrics Inc.). First, lateral displacement of the channels due to thermal drift was compensated for using an image correlation algorithm, and then the average height around the center of the extracellular surface (approximately 20 pixels, corresponding to approximately 0.6 nm2) of the channel was measured. We used height transitions from 86 channels for the analysis. Baseline fluctuations in the raw data were removed (fig. S8) according to the calculations detailed in the Supplementary Materials.

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