Rat monoclonal anti-KDEL and rabbit monoclonal anti-GM130 antibodies were from Abcam (ab50601 and ab52649, respectively). Rabbit polyclonal anti-TRAPα antibodies were from Novus Biologicals (NBP1-86912). A mouse monoclonal antibody that recognizes a human proinsulin C-peptide–A-chain junction peptide (GSLQKRGIVE) was raised by Abmart. Mouse monoclonal anti-tubulin antibodies were from Sigma (T5168). Guinea pig polyclonal anti-porcine insulin antibodies were from Millipore. Rabbit polyclonal anti-AAT antibodies were from Dako (A001202-2). Rabbit polyclonal anti-HSP90 (no. 4874S), anti-eIF2 (no. 9722S), and anti–phospho-Ser51 eIF2 antibodies (no. 9721) were from Cell Signaling Technology. 35S-Met/Cys was from ICN. Dithiothreitol (DTT), protein A-agarose, digitonin, N-ethylmaleimide, and other chemical reagents were from Sigma-Aldrich. Gradient SDS–polyacrylamide gel electrophoresis (SDS-PAGE; 4 to 12%) was performed using NuPAGE gels from Thermo Fisher Scientific. Met/Cys-deficient Dulbecco’s modified Eagle’s medium and all other tissue culture reagents were from Invitrogen. A cDNA encoding Myc epitope–tagged human TRAPα was synthesized by Integrated DNA Technologies and was subcloned into the pTARGET mammalian–expressing vector (Promega).

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