Dauer arrest assays were performed at 25°C as previously described (7). Crude dauer pheromone was isolated as described (12), and dauer pheromone assay was performed as described (4). Briefly, well-fed, gravid hermaphrodites were transferred to 35-mm assay plates containing 0, 2, or 10 μl of pheromone for a 2-hour synchronized egg lay at 20°C. Gravid animals were removed, and plates were transferred to 25°C and scored for dauers after an 80-hour incubation. For each experiment, 10 plates of animals of each genotype were scored for each of the three pheromone concentrations. The experiment was performed twice.

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