HFF cells CCD-1070Sk (American Type Culture Collection CRL-2091) were cultured in DMEM high-glucose medium (Gibco, catalog no. 21063-029) in the absence of phenol red and supplemented with 10% fetal bovine serum (FBS; Axenia Biologix LLC ), sodium pyruvate (1 mM, Gibco), MEM nonessential amino acids (1×; Gibco), and penicillin/streptomycin (100 U/ml and 100 μg/ml; Gibco), herein referred to as normal culture medium. The cells were grown at 37°C with 5% CO2. Fibroblasts with stably expressing eGFP-paxillin (fused at the C terminus) were prepared as previously described (15).

pKO mouse kidney fibroblasts rescued with either αv, β1, or both αv and β1 integrin subunits were a gift from R. Fässler (Max Planck Institute Martinsried) (22). Cells were cultured on fibronectin-coated plastic (5 μg/ml; Corning, diluted in PBS and incubated at 37°C for 1 hour) in normal culture medium described above. pKO-β1 cells in particular were sensitive to the quality of fibronectin coating; thus, a minimum of 1 and 4 ml of the diluted fibronectin solution (5 μg/ml) were used per well for a six-well and 10-cm dish, respectively. Cells were grown at 37°C with 5% CO2.

WT and vin−/− MEFs were a gift from K. Rothenberg and B. Hoffman (Duke University) (28). Cells were cultured on tissue culture plastic in normal culture medium at 37°C and 5% CO2.

U2OS cells were a gift from M. Franklin and J. Liphardt (Stanford University). Cells were cultured on tissue culture plastic in normal culture medium at 37°C and 5% CO2.

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