The MazF treatment was performed in 10-μl volume with 500 ng of mRNA and 10 U of MazF at 37°C for 30 min. The digested mRNA and nondigested mRNA were subjected to reverse transcription using 5× TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal, TransGen Biotech, AT341-02). TB Green Premix Ex Taq II (Tli RNaseH Plus, Takara Bio Inc., code no. RR820A) was used on BIO-CFX96 Touch.

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