The MazF treatment was performed in 10-μl volume with 500 ng of mRNA and 10 U of MazF at 37°C for 30 min. The digested mRNA and nondigested mRNA were subjected to reverse transcription using 5× TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal, TransGen Biotech, AT341-02). TB Green Premix Ex Taq II (Tli RNaseH Plus, Takara Bio Inc., code no. RR820A) was used on BIO-CFX96 Touch.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.